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Catalog Number | orb6506 |
---|---|
Category | Antibodies |
Description | NFKB p65 (phospho-Thr435) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from human NFKBp65 around the phosphorylation site of Thr435 (EG(p-T)LS) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, ICC=1:100, Flow-Cyt=2ug/Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 61 kDa |
Target | phospho-NFKB p65 (Thr435) |
UniProt ID | Q04206 |
RRID | AB_10940587 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | NF-kB p65 (phospho T435); p-NF-kB p65 (phospho T43 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry of Mouse spleen.Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Thr435) antibody; Dilution:2μg /10^6 cells.
Flow cytometry of MCF7.Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Thr435) antibody; Dilution:2μg /10^6 cells.
ICC analysis of Hela cell using phospho-NFKB p65 (Thr435) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, WB | |
Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Biotin |
FC, ICC, IF | |
Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
PE |
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