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Catalog Number | orb6507 |
---|---|
Category | Antibodies |
Description | NFKB p65 (phospho-Thr254) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Bovine, Canine, Porcine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from human NFKBp65 around the phosphorylation site of Thr254 (FR(p-T)PP) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=2ug/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 61 kDa |
Target | phospho-NFKB p65 (Thr254) |
UniProt ID | Q04206 |
RRID | AB_10939059 |
Storage | Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | NF-kB p65 (phospho T254); p-NF-kB p65 (phospho T25 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of HL 60 cells using NFKB p65 (phospho-Thr254) antibody.
Western blot analysis of mouse lung tissue (Line 1) , mouse spleen cell lysate (Line 2) using NFKB p65 (phospho-Thr254) antibody.
Western blot analysis of extracts from spleen tissue(mo) using Phospho-NFKB p65(Thr254) antibody.
IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC | |
Bovine, Canine, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PerCP/Cy7 |
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