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Catalog Number | orb342330 |
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Category | Assays and Kits |
Description | NFkB Antibody Sampler Kit |
Tested applications | ELISA, WB |
Dilution range | ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Conjugation | Unconjugated |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Alternative names | Anti-NFKB p50, Anti-NFKB p65, Rabbit Anti-NFKB cRe Read more... |
Note | For research use only |
Application notes | Each kit contains one unit of the following products: Anti-NFKB p50 (NFKB1) (RABBIT) Antibody (100 µL) Anti-NFKB p65 (Rel A) (RABBIT) Antibody (100 µL) Anti-NFKB cRel (RABBIT) Antibody (100 µL) Anti-IKBα C-terminal (RABBIT) Antibody (100 µL) Anti-RABBIT IgG (H&L) (GOAT) Peroxidase Conjugated Antibody (100 µg) CONTROL PEPTIDE for Anti-NFKB p50 (NFKB1) (RABBIT) Antibody (50 µg) CONTROL PEPTIDE for Anti-NFKB p65 (Rel A) (RABBIT) Antibody (50 µg) CONTROL PEPTIDE for Anti-NFKB cRel (RABBIT) Antibody (50 µg) CONTROL PEPTIDE for Anti-IKBα C-terminal (RABBIT) Antibody ( (50 µg) |
Expiration Date | Please enquire. |
Western blot of HeLa cell extract using Anti-NFKB p50 (NFKB1) Antibody orb750584. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
Western blot of HeLa cell extract using p/n orb750589 Anti-NFKB cRel (RABBIT) Antibody (100 µL). All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
Western blot of HeLa cell extract. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed with anti NFkB antibody orb750586. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
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