NFκB-p105/p50 (phospho Ser337) rabbit pAb

• Catalog Number: orb764341
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NF kappa B-p105/p50 (phospho-Ser337)
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Synthesized phospho-peptide around the phosphorylation site of human NFκB-p105/p50 (phospho Ser337)
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 105+50
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-NFKB1 antibody, anti-Nuclear factor NF-kappa-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-NFκB-p105/p50 (S337) Polyclonal Antibody diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast cancer, using NF-κB p105/p50 (Phospho-Ser337) Antibody. The picture on the right is blocked with the NF-κB p105/p50 (Phospho-Ser337) peptide.