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Catalog Number | orb11120 |
---|---|
Category | Antibodies |
Description | NFATc1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P |
Predicted Reactivity | Mouse |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human NFATc1 (601-716/716 aa) |
Concentration | 1mg/ml |
Dilution range | IHC-P=1:100-500, Flow-Cyt=1ug/test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 101 kDa |
Target | NFAT2 |
UniProt ID | O95644 |
RRID | AB_10749499 |
Storage | Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | NFAT2; MGC138448; NF ATc; NF ATc1; NFAT 2; NFATc1; Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse lymphoma tissue (dilution at:1:200) using NFATc1 antibody
IHC-P of mouse hippocampal tissue using NFATc1 antibody
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Anti
Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-NFAT2 antibody (orb11120) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Canine, Guinea pig, Mouse | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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