NF2 (phospho-S518) antibody

• Catalog Number: orb345404
• Category: Antibodies
• Description: NF2 (phospho-S518) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,200,000, IHC: User Optimized, WB: 1:500- 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product). The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho-specific polyclonal antibody is specific for phosphorylated pS518 of human NF2 (neurofibromatosis 2 gene product). Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Cross-reactivity with NF2 (Merlin) occurs in mouse tissue. Reactivity with NF2 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P35240
• NCBI: NP_000259
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-NF2 pS518 antibody, rabbit anti-Merlin
• Note: For research use only
• Application notes: This phospho-specific polyclonal antibody was tested by immunoblotting and ELISA. By ELISA, the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse NF2 (Merlin). Although not tested, this antibody is likely functional in Immunofluorescence, immunohistochemistry, and immunoprecipitation.
• Expiration Date: 12 months from date of receipt.

Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4 nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (orb347654) preceded color development.