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Catalog Number | orb1525422 |
---|---|
Category | Antibodies |
Description | NF110 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 844 and C-terminus |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NF110 |
UniProt ID | Q12906 |
NCBI | NP_036350.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | nuclear factor associated with dsRNA; NF110b; NF90 Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human NF110 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF110 antibody orb1525422 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of mouse NF110 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF110 antibody orb1525422 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human NF110 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF110 antibody orb1525422 used for IP at 6 µg per reaction.
Detection of human NF110 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NF110 (Cat. No. orb1525422) used at a dilution of 1: 5, 000 (0.2 µg/ml). Detection: DAB
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Zebrafish | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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