NF-L Rabbit Polyclonal Antibody

• Catalog Number: orb1816976
• Category: Antibodies
• Description: NF-L Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human NF-L (2-543/543aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: NEFL
• UniProt ID: P07196
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Neurofilament L; Neurofilament 68; Neurofilament t
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): U251. Primary Antibody (green line): Rabbit Anti-NF-L antibody (orb1816976), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Polyclonal Antibody, Unconjugated (orb1816976) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Polyclonal Antibody, Unconjugated (orb1816976) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Polyclonal Antibody, Unconjugated (orb1816976) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Polyclonal Antibody, Unconjugated (orb1816976) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Polyclonal Antibody, Unconjugated (orb1816976) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Polyclonal Antibody, Unconjugated (orb1816976) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Polyclonal Antibody, Unconjugated (orb1816976) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Polyclonal Antibody, Unconjugated (orb1816976) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NF-L Polyclonal Antibody, Unconjugated (orb1816976) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, orb868605), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NF-L Polyclonal Antibody, Unconjugated (orb1816976) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, orb868605), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NF-L Polyclonal Antibody, Unconjugated (orb1816976) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, orb868605), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NF-L Polyclonal Antibody, Unconjugated (orb1816976) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, orb868605), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NF-L Polyclonal Antibody, Unconjugated (orb1816976) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, orb868605), DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Lane 3: Rat Cerebellum tissue lysates, Lane 4: Human U251 cell lysates, Primary: Anti-NF-L (orb1816976) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 62 kDa.