NF-L Mouse Monoclonal Antibody

• Catalog Number: orb1152079
• Category: Antibodies
• Description: NF-L Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5H1
• Tested applications: ELISA
• Predicted Reactivity: Human
• Reactivity: Mouse, Rat
• Isotype: IgG1
• Immunogen: Recombinant Human NF-L Protein (1-543/543aa)
• Antibody Type: Primary Antibody
• Dilution range: ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: NEFL
• UniProt ID: P07196
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M PBS (pH7.4).
• Alternative names: Neurofilament L; Neurofilament 68; Neurofilament t
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized Human NF-L protein, His Tag at 2 µg/mL (100 µl/well) can bind Mouse Anti-Human NF-L Antibody, the EC50 is 87.36 ng/ml.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152079) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152079) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152079) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152079) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Recombinant Human NF-L Protein at 100 ng, Primary: Mouse Anti-NF-L Monoclonal Antibody at 1/1000 dilution (Cat.orb1152079), Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 61.4kD, Observed band size: 68/44kD.