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Catalog Number | orb764243 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to NF kappa B-p65 (phospho-Ser276) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser276. AA range:249-298 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 60 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-RELA antibody, anti-NFKB3 antibody, anti-Tran Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, NFκB-p65 (phospho Ser276) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, NFκB-p65 (phospho Ser276) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, NFκB-p65 (phospho Ser276) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, NFκB-p65 (phospho Ser276) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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