NF-κB p65/RelA Antibody

• Catalog Number: orb1294326
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NF-kB p65
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:1000-1:2000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:300
• Conjugation: Unconjugated
• Target: NF-κB p65 / RelA
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-NF-kB p65 antibody at 1/1000 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294326 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NF-KB p65 with orb1294326 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NF-KB p65 with orb1294326 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.