Neuroligin 1 Rabbit Polyclonal Antibody

• Catalog Number: orb100471
• Category: Antibodies
• Description: Neuroligin 1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Neuroligin 1 (701-800/863aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 87 kDa
• Target: NLGN1
• UniProt ID: Q8N2Q7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NLG 1; KIAA1070; MGC45115; NLG1; Neuroligin-1; NLG
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): U251 (fixed with 70% ethanol for 5 min at -20℃). Primary Antibody (green line): Rabbit Anti-Neuroligin 1 antibody (orb100471), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test.

Blank control (blue): Mouse brain cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Neuroligin 1/PE Conjugated antibody, Dilution: 5 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/PE (orange), used under the same conditions.

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-Neuroligin 1 (orb100471) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 87 kD, Observed band size: 87 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Neuroligin 1 (orb100471) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 87 kD, Observed band size: 87 kD.

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Neuroligin 1 (orb100471) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 87 kD, Observed band size: 87 kD.