Neurofilament L / NF L

• Catalog Number: orb1294399
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Neurofilament L
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit-IgG
• Immunogen: Recombinant fragment corresponding to Human Neurofilament L. Encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:1000-1: 2000 Immunofluorescence 1:200-1:500
• Conjugation: Unconjugated
• Target: Neurofilament L / NF L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Neurofilament-L/NF-L antibody at 1/1000 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294399 overnight at 4°C.

Immunofluorescent analysis. Sample: primary cortical neurons; NF-L antibody (orb1294399): 1-250; Red: Tuj1 antibody; Anti-rabbit 488: 1-500; Fixed: 4% paraformaldehyde at RT for 20 min.