Neurofilament Antibody (-Light) / NF-L / NEFL

• Catalog Number: orb749717
• Category: Antibodies
• Description: This mAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NR-4
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the Neurofilament antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Neurofilament antibody is available for research use only.
• UniProt ID: P07196
• Storage: Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Neurofilament antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament antibody (NR-4).

IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with Neurofilament antibody (NR-4).

SDS-PAGE Analysis of Purified, BSA-Free Neurofilament Antibody (clone NR-4). Confirmation of Integrity and Purity of the Antibody.

Immunofluorescent staining of permeabilized human HEK293 cells with Neurofilament antibody (clone NR-4, green) and Phalloidin (red).

Flow cytometry testing of permeabilized human HEK293 cells with Neurofilament antibody (clone NR-4); Red=isotype control, Blue=Neurofilament antibody.