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Catalog Number | orb1151476 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to NeuN |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | NeuN/288R |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Recombinant full-length human RNA binding protein fox-1 homolog 3 protein was used as the immunogen for the recombinant NeuN antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml,Western blot:1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant NeuN antibody is available for research use only. |
UniProt ID | A6NFN3 |
Storage | Aliquot the recombinant NeuN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant NeuN antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human cerebellum tissue with recombinant NeuN antibody (clone NeuN/288R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cerebrum tissue with recombinant NeuN antibody (clone NeuN/288R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human brain tissue lysate using recombinant NeuN antibody (clone NeuN/288R).
Western blot testing of human HeLa cell lysate using recombinant NeuN antibody (clone NeuN/288R).
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-P | |
Bee, Equine, Mouse, Sheep | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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