NeuN Antibody / Fox3

• Catalog Number: orb1151476
• Category: Antibodies
• Description: Rabbit monoclonal antibody to NeuN
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: NeuN/288R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant full-length human RNA binding protein fox-1 homolog 3 protein was used as the immunogen for the recombinant NeuN antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot:1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant NeuN antibody is available for research use only.
• UniProt ID: A6NFN3
• Storage: Aliquot the recombinant NeuN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant NeuN antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cerebellum tissue with recombinant NeuN antibody (clone NeuN/288R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebrum tissue with recombinant NeuN antibody (clone NeuN/288R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human brain tissue lysate using recombinant NeuN antibody (clone NeuN/288R).

Western blot testing of human HeLa cell lysate using recombinant NeuN antibody (clone NeuN/288R).