You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1294409 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Nestin antibody (NES) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the N-terminus region. |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200 |
Conjugation | Unconjugated |
Target | Nestin / NES |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-Nestin antibody at 1/1000 dilution, Lysates at 30 μg per lane.
Sample: Neuron 2A; Nestin antibody (orb1294409): 1-1000.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294409 anti-Nestin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-P | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating