NENF Antibody

• Catalog Number: orb1239386
• Category: Antibodies
• Description: NENF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: NENF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF.The immunogen is located within the last 50 amino acids of NENF.
• Concentration: 1 mg/mL
• Dilution range: NENF antibody can be used for detection of NENF by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NENF
• UniProt ID: Q9UMX5
• NCBI: NP_037481
• Storage: NENF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: NENF Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NENF Antibody: CIR2, SPUF, SCIRP10, CIR2, Neudesin
• Note: For research use only
• Application notes: NENF antibody can be used for detection of NENF by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of NENF in rat kidney tissue with NENF antibody at 10 µg/mL.

Immunofluorescence of NENF in rat kidney tissue with NENF antibody at 20 µg/mL.