NEK7 Antibody

• Catalog Number: orb1249003
• Category: Antibodies
• Description: NEK7 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NTLANFRIEKK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35kDa band observed in lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_598001.1). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thyroid. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NEK7
• UniProt ID: Q8TDX7
• NCBI: NP_598001.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NEK7, NIMA (never in mitosis gene a)-related kinas
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35kDa band observed in lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_598001.1). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thyroid. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249003 (0.05 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249003 (3.75 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.