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Catalog Number | orb1249002 |
---|---|
Category | Antibodies |
Description | NEK7 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine |
Reactivity | Human, Mouse, Rat |
Immunogen | The immunogen for this antibody is: CINPDPEKRPDVT |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30-33kDa band observed in Human Lung and Testis lysates and in Mouse and Rat Lung and Testis lysates (calculated MW of 34.6kDa according to Human NP_598001.1 and 34.5kDa according to Mouse NP_067618.1). Recommended concentration: 0.1-0.3ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | NEK7 |
UniProt ID | Q8TDX7 |
NCBI | NP_598001.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | NEK7, NIMA (never in mitosis gene a)-related kinas Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30-33kDa band observed in Human Lung and Testis lysates and in Mouse and Rat Lung and Testis lysates (calculated MW of 34.6kDa according to Human NP_598001.1 and 34.5kDa according to Mouse NP_067618.1). Recommended concentration: 0.1-0.3ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1249002 (0.1 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1249002 (0.3 ug/ml) staining of Mouse Lung (A), Mouse Testis (B), Rat Lung (C) and Rat Testis (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Mouse, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
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