NEDD8 Protein, Human, Recombinant (His & SUMO)

• Catalog Number: orb1961194
• Category: Proteins
• Description: Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation. NEDD8 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis and SUMO tag. The predicted molecular weight is 24 KDa and the accession number is Q15843.
• Tag: N-6xHis, SUMO
• Purity: 98.00%
• MW: 24 KDa (reducing condition)
• UniProt ID: Q15843
• Protein Sequence: Met1-Gly76
• Expression System: E. coli
• Biological Origin: Human
• Biological Activity: Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation. NEDD8 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis and SUMO tag. The predicted molecular weight is 24 KDa and the accession number is Q15843.
• Expression Region: Met1-Gly76
• Storage: -20°C
• Note: For research use only
• Application notes: Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
• Expiration Date: 6 months from date of receipt.