NEDD4L Antibody

• Catalog Number: orb1256003
• Category: Antibodies
• Description: NEDD4L Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 836-955 of human NEDD4L (NP_056092.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 113kDa
• Target: NEDD4L
• UniProt ID: Q96PU5
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: E3 ubiquitin-protein ligase NEDD4-like, 632-, NEDD
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using NEDD4L antibody (orb1256003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using NEDD4L antibody (orb1256003) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using NEDD4L antibody (orb1256003) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using NEDD4L antibody (orb1256003) at dilution of 1:100. Blue: DAPI for nuclear staining.