NDUFS2 Antibody

• Catalog Number: orb1535225
• Category: Antibodies
• Description: NDUFS2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NDUFS2. The exact sequence is proprietary.
• Concentration: 0.89 mg/ml
• Dilution range: ICC (1:100 - 1:1000), IHC, IHC-P (7.5 µg/ml), WB (1:1000 - 1:10000)
• Conjugation: Unconjugated
• Target: NDUFS2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.1 M Tris, pH 7, 0.1 M Glycine, 20% Glycerol, 0.01% Thimerosal. 0.1 M Tris, pH 7, 0.1 M Glycine, 20% Glycerol, 0.01% Thimerosal
• Alternative names: NDUFS2, Complex I 49kDa subunit, Complex I-49kD, C
• Note: For research use only
• Application notes: Further information: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
• Expiration Date: 12 months from date of receipt.

Anti-NDUFS2 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

NDUFS2 antibody detects NDUFS2 protein at mitochondria by immunofluorescent analysis. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. NDUFS2 protein stained by NDUFS2 antibody diluted at 1:500.

Sample (30 ug of whole cell lysate). A:293T, B: A431, C: JurKat, D: Raji. 10% SDS PAGE. NDUFS2 antibody diluted at 1:1000.