NDUFA9 Rabbit Polyclonal Antibody

• Catalog Number: orb2265
• Category: Antibodies
• Description: NDUFA9 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NDUFA9 (131-230/377aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: NDUFA9
• UniProt ID: Q16795
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: mitochondrial; CI-39kD; Complex I subunit NDUFA9;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-NDUFA9/AF647 Conjugated antibody, dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF647. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-NDUFA9 (orb2265) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.