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Catalog Number | orb1671607 |
---|---|
Category | Antibodies |
Description | NCR3LG1 Antibody |
Clonality | Recombinant |
Clone Number | 17B1.3 |
Tested applications | Crystallography, FC, IF |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | NCR3LG1 |
UniProt ID | Q68D85 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | B7H6, B7 homolog 6, Natural cytotoxicity triggerin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry using the Anti-B7-H6 antibody 17B1.3. Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 17B1.3 (orb1671607 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed K562 cells with anti-B7-H6 antibody 17B1.3. Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric r version of 17B1.3 (orb1671607) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671607- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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