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Catalog Number | orb1520737 |
---|---|
Category | Antibodies |
Description | NCLN Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 375 and 425 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:10,000 - 1:25,000; IP - 20 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NCLN |
UniProt ID | Q969V3 |
NCBI | Q969V3.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | NET59; nicalin; nicalin homolog; nicastrin-like pr Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse NCLN by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody (orb1520737) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human NCLN by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, LNCaP, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody (orb1520737) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human NCLN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCLN antibody (orb1520737) used for IP at 1 µg per reaction.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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