NCE2 Peptide

Catalog Number: orb1236265

Catalog Number	orb1236265
Category	Proteins
Description	NCE2 Peptide
Tested applications	Blocking
Form/Appearance	Lyophilized
Target	UBE2F
UniProt ID	Q969M7
NCBI	NP_542409.1
Storage	Store at -20°C. When reconstituted minimize freezing and thawing.
Buffer/Preservatives	NCE2 peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.
Alternative names	NCE2, NCE2, NEDD8-conjugating enzyme UBE2F, NEDD8 Read more...
Note	For research use only
Application notes	NCE2 Peptide is used for blocking the activity of NCE2 antibody (catalog number 42-142). The NCE2 antibody ships at 0.5 mg/mL and the NCE2 Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
Expiration Date	6 months from date of receipt.