NCAM1 Antibody

• Catalog Number: orb1671664
• Category: Antibodies
• Description: NCAM1 Antibody
• Clonality: Recombinant
• Clone Number: NCAM12.19
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: This antibody was raised against human NCAM/CD56
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: NCAM1
• UniProt ID: P13591
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Ncam, NCAM1, HCD56, NKH-1, Neural Cell Adhesion Mo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of Kelly cells using anti-CD56 (NCAM12.19). Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric mouse IgG version of NCAM12.19 (orb1671664) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom. 1- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD56 antibody NCAM12.19, human brain cerebellum(A) and cerebral cortex(B) tissue lysate (35 ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of NCAM12.19 (orb1671664) at 0.03 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.