NCAM1 Antibody (C-term)

• Catalog Number: orb1788087
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: 796-830 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:25, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 94574 Da
• Target: This NCAM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 796-830 amino acids from the C-terminal region of human NCAM1.
• UniProt ID: P13591
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Neural cell adhesion molecule 1, N-CAM-1, NCAM-1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. astroglioma section using NCAM1 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Flow cytometric analysis of SH-SY5Y cells using NCAM1 Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

All lanes: Anti-NCAM1 Antibody (C-term) at 1:1000 dilution. Lane 1: HT-1080 whole cell lysates. Lane 2: SW480 whole cell lysates. Lane 3: human cerebellum lysates. Lane 4: human brain lysates. Lane 5: rat brain lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 95 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.