NCAM / CD56 Antibody

• Catalog Number: orb749710
• Category: Antibodies
• Description: This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NCAM1/795
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the CD56 antibody.
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD56 antibody is available for research use only.
• UniProt ID: P13591
• Storage: Store the CD56 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD56 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human HCT116 cell lysate using CD56 antibody. Predicted molecular weight: ~110 kDa (soluble fragment), ~120/125 kDa (GPI-anchored), 140/180 kDa (transmembrane isoforms).

IHC: FFPE human pancreas tested with CD56 antibody (NCAM1/795).

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with CD56 antibody.

SDS-PAGE Analysis of Purified, BSA-Free CD56 Antibody (clone NCAM1/795). Confirmation of Integrity and Purity of the Antibody.