NCAM / CD56 Antibody

• Catalog Number: orb1826023
• Category: Antibodies
• Description: This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NCAM/7523
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: A recombinant partial protein (within amino acids 400-650) from the human protein was used as the immunogen for the CD56 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD56 antibody is available for research use only.
• UniProt ID: P13591, P13592
• Storage: Aliquot the CD56 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human brain tissue lysate with CD56 antibody (clone NCAM/7523). Predicted molecular weight: ~110 kDa (soluble fragment), ~120/125 kDa (GPI-anchored), 140/180 kDa (transmembrane isoforms).

IHC staining of FFPE human colon tissue with CD56 antibody (clone NCAM/7523). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.