Napsin A Antibody

• Catalog Number: orb606457
• Category: Antibodies
• Description: Napsin-A is a protein that in humans is encoded by the NAPSA gene. The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [Wiki]
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rNAPSA/1239
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant fragment from the human protein (amino acids 189-299) was used as the immunogen for the recombinant Napsin A antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Napsin A antibody is available for research use only.
• UniProt ID: O96009
• Storage: Store the recombinant Napsin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Napsin A antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human lung adenocarcinoma with recombinant Napsin A antibody (clone rNAPSA/1239). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Napsin A antibody (clone rNAPSA/1239) as confirmation of integrity and purity.