Napsin A antibody (Biotin)

• Catalog Number: orb750320
• Category: Antibodies
• Description: Mouse monoclonal antibody against Napsin A conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NAPSA/3308
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids 189-299 from the human protein were used as the immunogen for the Napsin A antibody.
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Biotin
• Formula: 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Napsin A antibody is available for research use only.
• UniProt ID: O96009
• Storage: Store the Napsin A antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term).
• Buffer/Preservatives: 0.1 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Napsin A antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lung adenocarcinoma with biotin-conjugated Napsin A antibody (clone NAPSA/3308). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma with biotin-conjugated Napsin A antibody (clone NAPSA/3308). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Napsin A antibody (clone NAPSA/3308). These results demonstrate the foremost specificity of the NAPSA/3308 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Napsin A antibody (clone NAPSA/3308) as confirmation of integrity and purity.