NANOG Antibody

• Catalog Number: orb1247228
• Category: Antibodies
• Description: NANOG Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DLFLNYSMNMQPED
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1ug/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB06860.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NANOG
• UniProt ID: Q9H9S0
• NCBI: NP_079141.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NANOG, Nanog homeobox, homeobox transcription fact
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1ug/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB06860.
• Expiration Date: 12 months from date of receipt.

orb1247228 (0.05 ug/ml) staining of HeLa (nuclear) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247228 (5 ug/ml) as the reporter antibody.

orb1247228 (2 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.