NANOG Antibody

• Catalog Number: orb1239398
• Category: Antibodies
• Description: NANOG Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.The immunogen is located within amino acids 130 - 180 of NANOG.
• Concentration: 1 mg/mL
• Dilution range: NANOG antibody can be used for detection of NANOG by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NANOG
• UniProt ID: Q9H9S0
• NCBI: EAW88651
• Storage: NANOG antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: NANOG Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NANOG Antibody: Homeobox protein NANOG, Homeobox t
• Note: For research use only
• Application notes: NANOG antibody can be used for detection of NANOG by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of NANOG in human spleen tissue with NANOG antibody at 5 µg/mL.

Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 µg/mL.