Cart summary

You have no items in your shopping cart.

NALP3/CIAS1 Rabbit Polyclonal Antibody

Catalog Number: orb182473

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb182473
CategoryAntibodies
DescriptionNALP3/CIAS1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityMouse
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Cryopyrin (551-630/1036aa)
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
Form/AppearanceLiquid
ConjugationUnconjugated
MW114 kDa
TargetNLRP3
UniProt IDQ96P20
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesLRR and PYD domains-containing protein 3; AGTAVPRL
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
NALP3/CIAS1 Rabbit Polyclonal Antibody

Blank control (black line): Raw264.7. Primary Antibody (green line): Rabbit Anti-NALP3/CIAS1 antibody (orb182473), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

NALP3/CIAS1 Rabbit Polyclonal Antibody

Sample: Lung (Rat) Lysate at 40 ug, Colon carcinoma (Human) Lysate at 40 ug, Primary: Anti-NALP3/CIAS1 (orb182473) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 114 kD, Observed band size: 114 kD.

NALP3/CIAS1 Rabbit Polyclonal Antibody

Tissue/Cell: Rat tracheal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cryopyrin Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • NALP3/CIAS1 Rabbit Polyclonal Antibody [orb156462]

    IF,  IHC-Fr,  IHC-P,  WB

    Canine, Mouse, Rat

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • NALP3/CIAS1 Rabbit Polyclonal Antibody [orb500878]

    IF,  IHC-Fr,  IHC-P

    Canine, Equine, Porcine, Sheep

    Human, Mouse, Rabbit, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • NALP3/CIAS1 Rabbit Polyclonal Antibody [orb783044]

    WB

    Human

    Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • NALP3/CIAS1 Rabbit Polyclonal Antibody [orb500879]

    IF,  IHC-Fr,  IHC-P

    Mouse, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 50 μl, 100 μl
  • NALP3/CIAS1 Rabbit Polyclonal Antibody (PerCP-Cy5.5) [orb2443823]

    IF

    Canine, Equine, Porcine, Sheep

    Human, Mouse, Rabbit, Rat

    Rabbit

    Polyclonal

    PerCP/Cy5.5

    100 μl