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Catalog Number | orb1294398 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Sodium Potassium ATPase |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the N-terminus region. |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:100-1:200 |
Conjugation | Unconjugated |
Target | NaK ATPase alpha 1 / ATP1A1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-Na/K ATPase antibody at 1/700 dilution, Lysates/proteins at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294398 overnight at 4°C.
Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294398 anti-Na/K ATPase antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling Na/K ATPase with orb1294398 at 1/100.