You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb345643 |
---|---|
Category | Antibodies |
Description | NAG-1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH. |
Concentration | 1.1mg/ml |
Dilution range | ELISA: 1:300,000, IHC: User Optimized, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q99988 |
NCBI | Q99988.3 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-NAG1 antibody, NAG 1, NAG-1, GDF15, MI Read more... |
Note | For research use only |
Application notes | This affinity purified NAG-1 antibody has been tested by ELISA, IHC, and western blotting of human and mouse NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific). Tissue: Human Colon at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background.
Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific). Tissue: Human Colon at 20X in colon tissue at pH 9. Negative control of human colon tissue pH9 is shown in background.
Western blot using Biorbyt's affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
IF, IH, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating