N-Cadherin antibody

• Catalog Number: orb1151804
• Category: Antibodies
• Description: Rabbit monoclonal antibody to N-Cadherin
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CDH2/6857R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Recombinant full-length human CDH2 protein was used as the immunogen for the recombinant N-Cadherin antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant N-Cadherin antibody is available for research use only.
• UniProt ID: P19022
• Storage: Aliquot the recombinant N-Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant N-Cadherin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human ovarian carcinoma tissue with recombinant N-Cadherin antibody (clone CDH2/6857R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of intercalated discs in FFPE human heart tissue with recombinant N-Cadherin antibody (clone CDH2/6857R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot analysis of human heart tissue lysate using recombinant N-Cadherin antibody (clone CDH2/6857R). Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).