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Catalog Number | orb344308 |
---|---|
Category | Antibodies |
Description | N-Acylmannosamine-1-Dehydrogenase antibody (Biotin) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IP, WB |
Reactivity | Bacteria |
Isotype | IgG |
Immunogen | N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ] |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:250 - 1:1,000 |
Form/Appearance | Lyophilized |
Purity | Anti-N-Acylmannosamine-1-Dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified N-Acylmannoseamine-1-Dehydrogenase [Recombinant from Pseudomonas]. Cross reactivity against N-Acylmannoseamine-1-Dehydrogenase from other sources is unknown. |
Conjugation | Biotin |
UniProt ID | P22441 |
NCBI | P22441.3 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat anti-N-Acylmannosamine-1-Dehydrogenase Antibo Read more... |
Note | For research use only |
Application notes | Anti-N-Acylmannosamine-1-Dehydrogenase antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of N-Acylmannoseamine-1-Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:35,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Biorbyt Goat anti N-acylmanosamino-1-Dehydrogenase antibody was used to detect purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT).
Western Blot of Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated. Lane 1: N-Acylmannoseamide 1-Dehydrogenase. Load: 50 ng per lane. Primary antibody: Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated 1:1000 overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 27.5 kDa, observed at 30 kDa for N-Acylmannoseamide 1-Dehydrogenase.
ELISA, IP, WB | |
Bacteria | |
Goat | |
Polyclonal | |
Biotin |
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