MZF1 Antibody / Myeloid zinc finger 1

• Catalog Number: orb1824628
• Category: Antibodies
• Description: Zinc finger genes that encode metal-binding proteins are transcriptional regulators of other genes. Myeloid zinc finger 1 (MZF-1), also designated zinc finger protein 42, and transcription factor ZBP-89, also designated zinc finger protein 148, belong to the Kruppel C2H2-type zinc-finger protein family. The gene encoding for the MZF-1 protein maps to chromosome 19q13.43 while the gene encoding for ZBP-89 is localized on chromosome 3q21. These proteins are nuclear proteins involved in the regulation of transcriptional events. MZF-1 regulates transcription during hemopoietic development and plays a role in myeloid cell differentiation. MZF-1 regulates the CD34 promoter in a tissue-specific manner. MZF-1 and FHL3 can form a complex of high molecular mass with other proteins in the nucleus. It is induced by retinoic acid and is primarily expressed in differentiating myeloid cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-MZF1-1E8
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: A recombinant partial protein sequence (within amino acids 37-128) from the human protein was used as the immunogen for the Myeloid zinc finger 1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Myeloid zinc finger 1 antibody is available for research use only.
• UniProt ID: P28698
• Storage: Aliquot the Myeloid zinc finger 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon carcinoma tissue with Myeloid zinc finger 1 antibody (clone PCRP-MZF1-1E8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human HeLa cells with Myeloid zinc finger 1 antibody (clone PCRP-MZF1-1E8) followed by goat anti-mouse IgG-CF488 (blue), Red = unstained cells.

SDS-PAGE analysis of purified, BSA-free Myeloid zinc finger 1 antibody (clone PCRP-MZF1-1E8) as confirmation of integrity and purity.

Analysis of a HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Myeloid zinc finger 1 antibody (clone PCRP-MZF1-1E8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.