MYL12A antibody

• Catalog Number: orb750488
• Category: Antibodies
• Description: MYL12A antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: Antiserum
• Immunogen: Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 70 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: 2.5 µg/ml, IP: 1:100, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Myosin pS19/pS20 antibody is directed against the regulatory light chain of smooth and non-muscle myosin. This antiserum is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
• Conjugation: Unconjugated
• UniProt ID: P19105
• NCBI: AAH16372.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Myosin pS19/pS20 Antibody, Myosin regu
• Note: For research use only
• Application notes: Anti-Myosin pS19/pS20 phospho specific polyclonal antibody was tested by ELISA and immunoblotting. Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes. The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional by immunohistochemistry and immunoprecipitation.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit Anti-Myosin pS19/pS20 antibody. Lane 1: HeLa whole cell lysate (p/n orb348668). Lane 2: HeLa whole cell lysate + smooth muscle recombinant phospho protein. Lane 3: HeLa whole cell lysate + regulatory light chain recombinant phospho protein. Load: 10 µg of whole cell lysate + 1.0 µg of recombinant protein. Primary antibody: Mysoin pS19/pS20 antibody at 1.0 µg/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for RLC.

Western Blot of Rabbit Anti-Myosin pS19/pS20 antibody. Lane 1: Smooth muscle recombinant non-phospho protein. Lane 2: Smooth muscle recombinant phospho protein. Lane 3: Regulatory light chain recombinant non-phospho protein. Lane 4: Regulatory light chain recombinant phospho protein. Load: 1.0 ug of recombinant non-phospho protein, 5.0 ug of recombinant phospho protein. Primary antibody: Mysoin pS19/pS20 antibody at 1.0 ug/ml overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for RLC.