Myeloperoxidase / MPO antibody

• Catalog Number: orb2276279
• Category: Antibodies
• Description: Myeloperoxidase / MPO antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-692M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2a, kappa
• Immunogen: Recombinant fragment of human MPO protein (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P05164
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: 4 kDa / 89 kDa myeloperoxidase; EC 1.11.1.7; EC1.1
• Note: For research use only
• Application notes: Positive Control: Spleen: Numerous granulocytes with strong MPO staining should be seen within the red pulp of the spleen. Negative Control: Colon: MPO immunostaining should be absent in all epithelial cells of the colon while MPO positive granulocytes may be seen within capillaries or in the stroma of the lamina propria. Cellular Localization: Lysozome Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Colon descendes mucosa In the normal colon MPO positive granulocytes occur within small capillaries and also the stroma of the lamina propria.

Numerous MPO positive intravascular and stromal granulocytes are seen in a squamous cell carcinoma of the oral cavity.

Spleen Numerous MPO positive granulocytes are usually seen within the red pulp of the spleen.