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Catalog Number | orb749929 |
---|---|
Category | Antibodies |
Description | Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for > 70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1014 |
Tested applications | IF, WB |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the Major Vault Protein antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This Major Vault Protein antibody is available for research use only. |
UniProt ID | Q14764 |
Storage | Store the Major Vault Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Major Vault Protein antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent staining of human A549 cells with Major Vault Protein antibody (green, clone 1014) and Reddot nuclear stain (red).
Western blot testing of HeLa cell lysate with Major Vault Protein antibody (clone 1014). Observed molecular weight: 104~110 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Major Vault Protein Antibody (clone 1014). Confirmation of Integrity and Purity of the Antibody.
IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
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