Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)

• Catalog Number: orb2302689
• Category: Antibodies
• Description: This Multi-rAb antibody targets Mouse IgG (H+L) in FC and IF. It is higly specific to mouse IgG and shows minimal reactivity for rabbit, human, pig and bovine IgGs.
• Species/Host: Goat
• Clonality: Recombinant
• Tested applications: FC, IF, WB
• Reactivity: Mouse
• Concentration: 0.5 mg/mL
• Dilution range: 1:200-1:1000 for IF and FC, 1:5000-1:20,000 for western blotting
• Form/Appearance: Liquid
• Purity: The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
• Conjugation: CoraLite® Plus 750
• Storage: Store at -20°C. Stable for one year after shipment.
• Buffer/Preservatives: PBS with 50% glycerol, 10 mg/mL rAlbumin, 0.1% Proclin-300, pH 7.4.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Beta Tubulin monoclonal antibody (isotype IgG2a). Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-ZNF174 monoclonal antibody (isotype IgG3). Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-EIF3E monoclonal antibody (isotype IgG1) at dilution of 1:50000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Catalase monoclonal antibody (isotype IgG1) at dilution of 1:10000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Beta Tubulin monoclonal antibody (isotype IgG2a) at dilution of 1:100000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-FAK monoclonal antibody (isotype IgG2c) at dilution of 1:5000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-ZNF174 monoclonal antibody (isotype IgG3) at dilution of 1:2000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with anti-B23, followed by Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302689, 1:500, red), nuclei stained with DAPI (cyan). Confocal images were acquired with a 63x oil objective and post-processed.

Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with mouse anti-B23 and rabbit anti-Lamin B1, followed by Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302689, 1:500, red) and Nano-Secondary srb2GCL555-1 (yellow). Confocal images were acquired with a 63x oil objective and post-processed. Device setting for orb2302689: Laser Ex max 764nm, filter Em max 775nm.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody ( Clone: 1E4C11 ) at dilution of 1:400 and Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302689), CL488-Phalloidin (green).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (Clone: UCHT4) and Mouse IgG2a Isotype Control. Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:500 for detection.