MUC6 Antibody / Gastric Mucin

• Catalog Number: orb749697
• Category: Antibodies
• Description: The MUC6 gastric mucin is a secreted glycoprotein that plays an essential role in epithelial cyto-protection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 is associated with Lewis type 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CLH5
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A synthetic peptide of the gastric Mucin 6 tandem repeat sequence was used as the immunogen for the MUC6 antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This MUC6 antibody is available for research use only.
• UniProt ID: Q6W4X9
• Storage: Store the MUC6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MUC6 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human gastric carcinoma stained with MUC6 antibody (clone CLH5). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE human gastric carcinoma with MUC6 antibody (clone CLH5). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC staining of glandular cells of FFPE normal human stomach tissue with MUC6 antibody (clone CLH5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.