MUC5AC Antibody

• Catalog Number: orb749692
• Category: Antibodies
• Description: Mucin 5AC glycoprotein (MUC5AC) is a HMW glycoprotein belonging to the superfamily of mucins. Mucins are produced by epithelial cells and can be divided into two families; secretory mucins and membrane bound mucins. MUC5AC is a mucus-forming, secreted mucin that is found in normal gastric and tracheo-bronchial mucosa, but absent from normal colon. MUC5AC expression is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CLH2
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A synthetic peptide of human MUC5AC tandem repeat was used as the immunogen for the MUC5AC antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This MUC5AC antibody is available for research use only.
• UniProt ID: P98088
• Storage: Store the MUC5°C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MUC5AC antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human gastric carcinoma with MUC5AC antibody (clone CLH2). Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to testing.

IHC testing of FFPE human gastric carcinoma with MUC5AC antibody (clone CLH2). Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to testing.

SDS-PAGE Analysis of Purified, BSA-Free MUC5AC Antibody (clone CLH2). Confirmation of Integrity and Purity of the Antibody.