MUC5AC antibody

• Catalog Number: orb2276597
• Category: Antibodies
• Description: MUC5AC antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-109M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P98088
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Apomucin Major Airway Glycoprotein, Mucin 5 subtyp
• Note: For research use only
• Application notes: Positive Control: Gastric mucosa: A strong cytoplasmic MUC5AC staining should be seen in the surface epithelium. Negative Control: Kidney: MUC5AC staining should be absent. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

MUC5AC negative Brunner gland.

Stomach antrum mucosa with strong MUC5AC staining of surface epithelial cells.

Stomach- Strong MUC5AC immunostaining in a gastric adenocarcinoma (100% of tumor cells).