MUC2 Antibody / Mucin 2

• Catalog Number: orb749685
• Category: Antibodies
• Description: Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This mAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MLP/842
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the MUC2 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This MUC2 antibody is available for research use only.
• UniProt ID: Q02817
• Storage: Store the MUC2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MUC2 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris-HCl buffer, pH 10 for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with MUC2 antibody (clone MLP/842).

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with MUC2 antibody (clone MLP/842).

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with MUC2 antibody (clone MLP/842).

SDS-PAGE analysis of purified, BSA-free MUC2 antibody (clone MLP/842) as confirmation of integrity and purity.