MUC1 Antibody

• Catalog Number: orb1672037
• Category: Antibodies
• Description: MUC1 Antibody
• Clonality: Recombinant
• Clone Number: Mc5
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Human breast epithelial mucin.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: MUC1
• UniProt ID: P15941
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD227, BEM, Breast epithelial mucin, Mucin-1, Brea
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed MCF7 cells with anti-Breast epithelial mucin antibody Mc5. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of Mc5 (orb1672037) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672037- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-breast epithelial mucin antibody Mc5. MCF7 cell lysate (35 ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric r version of Mc5 (orb1672037) at 2 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified breast epithelial mucin is 122.1kDa- but this protein is heavily glycosylated and lipidated at several positions leading to the observed running size.